| LIU Changan,YANG Guang,JIA Tingzhen.Stimulatory effect of low dose irradiation on immune functions of cord blood[J].Chinese Journal of Radiological Medicine and Protection,2002,22(4):277-280 |
| Stimulatory effect of low dose irradiation on immune functions of cord blood |
| Received:January 14, 2002 |
| DOI： |
| KeyWords:Low dose irradiation Cord blood T lymphocytes Interleukin 2Lymphokine activated killer cells |
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| Abstract:: |
| Objective To investigate the stimulatory effect of low dose irradiation on immune functions of cord blood and its clinical significance. Methods Freshly isolated lymphocytes and LAK cells from cord blood were irradiated with low dose gamma-rays.The expressions of the membrane molecules of T lymphocytes, the levels of IL-2 in the supernatant and the cytotoxicity of LAK cells against target tumor cells (K562, HL-60) were examined by using flow cytometry with direct immunofluorescence, a bioassay based on CTLL cells, which served as target cells, and a 3H-TdR release assay, respectively. Results (1) The proportion of CD3+, TCR, CD3+, CD4+, CD8+ cells increased significantly after low dose irradiation, with the most obvious enhancement noted in the 24 h experimental group.The ratio of CD4 to CD8 showed no significant changes.(2) The biological activity of IL-2 in the supernatant of cord blood mononuclear cells was enhanced increasingly after low dose irradiation.(3) There was no significant difference in cytotoxicity of LAK cells against target tumor cells between cord blood and peripheral blood (P >0. 05).There was significantly increased cytotoxicity of LAK cells from cord blood against target tumor cells irradiated with low dose gamma-rays as compared with those of non-irradiated (AllP <0.01). Conclusion It is suggested that expedition of maturation, activation, signal transduction and IL 2 secretion of T lymphocytes from cord blood, as well as the enhancement of cytotoxicity of cord blood LAK cells against target tumor cells, which may contribute to intensifying elimination of minimal residue disease, facilitating the reconstruction of immune functions and strengthening the graft-versus-leukemia response in cord blood transplantation, can be induced by low dose irradiation. |
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