| LI Jinying,ZHOU Hong,XIA Fang.The delayed effect of radiation on the gene expression of anion exchanger in erythrocyte membrane[J].Chinese Journal of Radiological Medicine and Protection,2002,22(4):275-276 |
| The delayed effect of radiation on the gene expression of anion exchanger in erythrocyte membrane |
| Received:October 20, 2001 |
| DOI： |
| KeyWords:Delayed effect of radiation Erythrocyte membrane Anion exchanger Gene expression |
| FundProject:总后卫生部专项基金拔款资助项目(98M081) |
| Author Name | Affiliation | | LI Jinying | Changhai Hospital, Shanghai 200433, China | | ZHOU Hong | Changhai Hospital, Shanghai 200433, China | | XIA Fang | 复旦大学遗传研究所 | | 翟永平 | Changhai Hospital, Shanghai 200433, China | | 张雨生 | Changhai Hospital, Shanghai 200433, China | | 韩风来 | Changhai Hospital, Shanghai 200433, China | | 章卫平 | Changhai Hospital, Shanghai 200433, China | | 刘本俶 | Changhai Hospital, Shanghai 200433, China |
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| Abstract:: |
| Objective To study the delayed effect of radiation on the gene expression of anion exchanger (band 3 protein) in erythrocyte membrane. Methods Two-step liquid culture of erythroid cell was conducted by isolating blasts freshly from patients with bone marrow form of acute radiation sickness (ARS) at the 10 th year after exposure to radiation and the band 3 gene expression in the culture cells was observed using B3 mRNA in situ hybridization.The expression level of band 3 gene in total RNA from peripheral blood mononuclear cells of ARS patients was analyzed by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).β-actin was used as the internal reference. Results Weak and uneven location of band 3 mRNA positive stain in the cultured late erythroblasts was shown by using in situ hybridization.The expression level of band 3 mRNA in half of the ARS patients was decreased. Conclusion The results suggest that the delayed effect of radiation might be exerted on band 3 mRNA transcription, resulting in lowered level of the transcription product and its weakened function, e.g.the decreased anion transport of anion exchanger, and prolonged course of erythropoiesis. |
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