| PENG Ruiyun,WANG Dewen,GAO Yabing.Studies on the changes and significance for heat shock protein 70 expression in angiogenesis in healing of single wounding and radiation-combined of wounding skin[J].Chinese Journal of Radiological Medicine and Protection,2002,22(4):272-274 |
| Studies on the changes and significance for heat shock protein 70 expression in angiogenesis in healing of single wounding and radiation-combined of wounding skin |
| Received:September 06, 2001 |
| DOI： |
| KeyWords:Wound healing Radiation Angiogenesis HSP70 mmunohistochemistry |
| FundProject:全军“九五”重点招标课题基金资助项目(96Z008) |
| Author Name | Affiliation | | PENG Ruiyun | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | WANG Dewen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | GAO Yabing | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 熊呈琦 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 秦全红 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 崔雪梅 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 杨红 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 谷庆阳 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 夏国伟 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 崔玉芳 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 陈浩宇 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 胡文华 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract:: |
| Objective To observe the expression of HSP70 in healing of single wounding and radiation-combined wounding of skin and investigate its significance in capillary regeneration. Methods Altogether 72 Wistar rats were subjected to excision of a circular full-thickness skin with the diameter of 1 5 cm on the dorsum.After wounded, these rats in radiation group were locally irradiated immediately with 25 Gy 60 Co γ-rays.The skin specimens were obtained on days 2, 5, 10, 15, 21 and 28 after injury and the expression of HSP70 was investigated by means of immunohistochemistry. Results HSP70 expression was positive in the plasma of endothelial cells of newly-formed capillaries on day 2, strong positive in lot of these cells on day 5, positive in part of endothelial cells on day 10, and decreased on day 15 after wounding in the single wounding group, whereas in the radiation combined wounding group HSP70 was slightly positive in the endothelial cells on day 5, positive on days 10-15, and negative after day 21. Conclusion HSP70 plays an important role in the course of capillary regeneration.It could accelerate angiogenesis.HSP70 expression decreased by radiation might be one of reasons for delayed healing of radiation-combined wound. |
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