| GONG Lizhong,SUN Shihong,CHENG Tianmin,et al.The effect of coinfusion of peripheral blood stem cells and stromal cells on hematopoietic reconstitution in lethally irradiated mice[J].Chinese Journal of Radiological Medicine and Protection,2002,22(4):266-268 |
| The effect of coinfusion of peripheral blood stem cells and stromal cells on hematopoietic reconstitution in lethally irradiated mice |
| Received:October 26, 2001 |
| DOI： |
| KeyWords:Peripheral blood stem cells Stromal cells Irradiation injury |
| FundProject:国家重点基础研究发展规划项目(“973”项目)基金资助项目(G1999054205) |
| Author Name | Affiliation | | GONG Lizhong | Institute of Combined Injury of PLA, Third Military Medical University, Chongqing 400038, China | | SUN Shihong | 第三军医大学新桥医院肝胆外科 | | CHENG Tianmin | Institute of Combined Injury of PLA, Third Military Medical University, Chongqing 400038, China | | 粟永萍 | Institute of Combined Injury of PLA, Third Military Medical University, Chongqing 400038, China | | 冉新泽 | Institute of Combined Injury of PLA, Third Military Medical University, Chongqing 400038, China | | 罗成基 | Institute of Combined Injury of PLA, Third Military Medical University, Chongqing 400038, China | | 郭朝华 | Institute of Combined Injury of PLA, Third Military Medical University, Chongqing 400038, China |
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| Abstract:: |
| Objective To study the effect of coinfusion of peripheral blood stem cells and stromal cells on hematopoietic reconstitution in lethally irradiated mice. Methods BALB/c mice irradiated with 60 Co γ-rays were treated with RBC infusion (control group), peripheral blood stem cells infusion (PBSCT group), or coinfusion of peripheral blood stem cells and bone marrow stromal cells (experimental group).Survival rate within 4 weeks, white blood cell count, bone marrow nucleated cells (BMNC), granulocyte-macrophage colony-forming unit (GM-CFU), fibroblast colony-forming unit (F-CFU) were observed. Results Survival rate, BMNC, GM-CFU, F-CFU counts were significantly higher in the experimental group than those of the control andPBSCT groups (P <0. 05).The lowest number of WBC was significantly higher (P <0.01) and WBC recovery was faster (P <0.01) in the experimental group. Conclusion Coinfusion of peripheral blood stem cells and stromal cells could accelerate hematopoietic reconstitution in lethally irradiated mice and the effect is better than that of peripheral blood stem cell infusion (transplantation) only. |
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