| XUE Wencheng,WANG Baoqin,YUAN Bin.Comet-electrophoresis assay as a method for determining radiosensitivities of tumor cells[J].Chinese Journal of Radiological Medicine and Protection,2002,22(4):263-265 |
| Comet-electrophoresis assay as a method for determining radiosensitivities of tumor cells |
| Received:September 26, 2001 |
| DOI： |
| KeyWords:Tumor cells Radiosensitivities Radiation therapy Comet-electrophoresis assay DNA damage |
| FundProject:国家攀登计划(B)基金资助项目(8545011) |
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| Abstract:: |
| Objective To explore the feasibility of applying comet-electrophoresis to determining the radiosensitivity of tumor cells. Methods The residual rates of DNA damage at 30 minute after 2 Gy gamma irradiation in four human tumor cell lines (WM9839, KB, LS-T-117, PC3M) were determined with the comet assay.The cell survival fraction of tumor cell after 2 Gy gamma ray-irradiation was determined with colonogenic assay. Results There were good correlations between cell survival fraction (SF2) and residual rate of DNA damage at 30 minute after 2 Gy gamma ray-irradiation in these four human tumor cell lines, separately. Conclusion The comet-electrophoresis assay may be used as a repaid and sensitive method for determining inherent radiosensitivities of tumor cells. |
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