| WAN Hong,GONG Shouliang,LIU Shuzheng.Effects of low dose radiation on signal transduction of neurons in mouse hypothalamus[J].Chinese Journal of Radiological Medicine and Protection,2002,22(4):260-262 |
| Effects of low dose radiation on signal transduction of neurons in mouse hypothalamus |
| Received:November 12, 2001 |
| DOI： |
| KeyWords:Low dose radiation Hypothalamus Signal transduction |
| FundProject:国家自然科学基金资助项目(395701884) |
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| Abstract:: |
| Objective Effects of low dose radiation on signal transduction of neurons in mouse hypothalamus were investigated. Methods In the present study competitive protein binding assay, radioimmunoassay, in situ hybridization and immunohistochemistry were used to observe the effects of whole-body irradiation with 75 mGy X-rays on the contents of cAMP and cGMP and the expressions of c-fos mRNA, Fos protein and proopiomelanocortin (POMC) mRNA in neurons of mouse hypothalamus. Results The results showed that cAMP content in mouse hypothalamus immediately increased significantly and reached the peak value at 15 min after irradiation, and then returned to near the sham-irradiation level 1 h after irradiation;the changes of cGMP content were basically opposite to those of cAMP content, while the changes of cAMP/cGMP ratio were basically consistent with those of cAMP content.The expression of c-fos mRNA in the neurons of hypothalamus appeared 15 min after irradiation;The expression of Fos protein reached its peak value 8 h after irradiation.The expression ofPOMC mRNA decreased significantly 1 h after irradiation. Conclusion These findings implicate that low dose radiation may potentiate the activity of the neurons in mouse hypothalamus, expedite their signal transduction, and down-regulate the functions of hypothalamus-pituitary-adrenocortical axis. |
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