| JIN Shunzi,LIU Shuzheng.Effect of whole-body X-irradiation on the expression of costimulatory molecules on immune cells[J].Chinese Journal of Radiological Medicine and Protection,2002,22(4):256-259 |
| Effect of whole-body X-irradiation on the expression of costimulatory molecules on immune cells |
| Received:September 20, 2001 |
| DOI： |
| KeyWords:X-irradiation Costimulatory molecules CD28 CTLA-4 B7-1 B7-2 |
| FundProject:国家自然科学基金资助项目(39570188) |
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| Abstract:: |
| Objective To study the effect of ionizing radiation on costimulatory molecules on immune cells. Methods Flow cytometry (FCM) was used to detect the changes in the expression of CD28/CTLA-4 of thymocytes and splenocytes after whole-body irradiation (WBI) with different doses of X-rays.Immunohistochemistry (IHC, ABC method) was used to detect the changes in the expression of B7-1 and B7-2 on peritoneal ma crophages after WBI with different doses of X-rays. Results The results showed that 0.075 Gy X-rays caused significant up regulation of CD28 on both thymocytes and splenocytes and down-regulation of CTLA-4 expression, which was more significant on the splenocytes.After 2 Gy irradiation the expression of CTLA-4 on both thymocytes and splenocytes was significantly up-regulated with down-regulation of CD28 expression, which was more significant on the splenocytes.The dose-effect studies showed that there was no change in the expression of CTLA-4 on the thymocytes 72 h after LDR, while 2 and 4 Gy caused apparent increase in the expression of CTLA-4(P <0.05, P <0.01).For splenocytes, LDR caused no changes in the expression of CTLA-4 4 h after irradiation, while higher doses could increase the expression of CTLA-4 significantly (P <0.05, P <0.01).The expression of B7-1 and B7-2 on peritoneal macrophages increased after both 0.075 Gy and 2 Gy X-rays.The increase in B7-2 expression preceded that of B7-1 expression after 0.075 Gy and lasted longer than after 2 Gy irradiation. Conclusion Up-regulation of expression of costimulatory molecules CD28 and B7 after whole-body X-irradiation may be an important factor involved in the enhancing effect of low dose radiation on immunity. |
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