| WANG Yanjiang,SU Yongping,AI Guoping,et al.Effect of combined radiation-burn injury on cell number,morphology and IL-12 gene expression of peritoneal macrophages in mice[J].Chinese Journal of Radiological Medicine and Protection,2002,22(3):192-194 |
| Effect of combined radiation-burn injury on cell number,morphology and IL-12 gene expression of peritoneal macrophages in mice |
| Received:November 19, 2001 |
| DOI： |
| KeyWords:Combined radiation-burn injury Immunological function Macrophage IL-12 |
| FundProject:国家自然科学基金资助项目(39670289);教育部高等学校骨干教师资助计划项目 |
| Author Name | Affiliation | | WANG Yanjiang | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University Chongqing 400038, China | | SU Yongping | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University Chongqing 400038, China | | AI Guoping | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University Chongqing 400038, China | | 冉新泽 | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University Chongqing 400038, China | | 刘晓宏 | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University Chongqing 400038, China | | 练燕 | 西南医院血液科 | | 郭朝华 | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University Chongqing 400038, China | | 胡川闽 | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University Chongqing 400038, China | | 程天民 | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University Chongqing 400038, China |
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| Abstract:: |
| Objective To investigate the effect of combined radiationburn injury on cell number,morphology and IL-12 gene expression of peritoneal macrophages (pMΦs) in mice.Methods At different time points after combined radiationburn injury(CRBI) or radiation injury(RI) pMΦs of mice were purified,counted and observed underlight microscope,and the IL-2 transcriptive level was measured with RTPCR.Results ①Compared with RI group,pMΦs decreased more quickly and seriously at early stage,but recovered better at late stage in CRBI group②Compared with the irradiation group,there was more necrocytosis in early stage in the CRBI group,but pMΦs in the CRBI group looked closer to normal③IL-12 p35 transcription levels were suppressed while that of p40 elevated in both CRBI and RI groups.However the transcriptive levels of p35 and p40 was higher in the CRBI group those in the RI group.Conclusion The effect of combined radiationburn injury on cell number,morphology and IL-12 gene expression of pMΦs are characterized as ‘worsening at early stage,lightening at late stage',implying the significance of early treatment of CRBI. |
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