| LIU Jianzhong,ZHOU Yuanguo,CHENG Tianmin.Phase-changes in cell cycle of wound tissue irradiated with 5.21 Gy soft X-rays[J].Chinese Journal of Radiological Medicine and Protection,2002,22(3):180-183 |
| Phase-changes in cell cycle of wound tissue irradiated with 5.21 Gy soft X-rays |
| Received:November 19, 2001 |
| DOI： |
| KeyWords:Soft X ray Local irradiation Wound healing Cell cycle |
| FundProject:国家重点基础研究发展规划“973”专项经费资助项目(G1999054205) |
| Author Name | Affiliation | | LIU Jianzhong | Molecular Biology Center, Research Institute of SurgeryDaping Hospital, Third Military Medical University, Chongqing 400042, China | | ZHOU Yuanguo | Molecular Biology Center, Research Institute of SurgeryDaping Hospital, Third Military Medical University, Chongqing 400042, China | | CHENG Tianmin | 第三军医大学预防医学系全军复合伤研究所 | | 周萍 | Molecular Biology Center, Research Institute of SurgeryDaping Hospital, Third Military Medical University, Chongqing 400042, China | | 刘霞 | Molecular Biology Center, Research Institute of SurgeryDaping Hospital, Third Military Medical University, Chongqing 400042, China | | 李平 | Molecular Biology Center, Research Institute of SurgeryDaping Hospital, Third Military Medical University, Chongqing 400042, China |
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| Abstract:: |
| Objective To study the phase changes in cell cycle of wound tissue which was locally irradiated with 5.21 Gy soft X rays.Methods Flow cytometry and PI staining were used to analyze cell cycle.Cell proliferation was determined with BrdU labeling.Results During 3 9 days after irradiation,the percentage of the G0/G1 phase cells in wound of the control side decreased while the percentage of S phase cells increased and reached the highest value on day 9.The percentage of G1/M phase cells also increased,and reached its peak on day 15.The percentage of G0/G1 phase cell increased in wound of the irradiation side and was higher than that of the control wound,meanwhile the percentages of S and G2/M cells were significantly lower than those of the control wound.In the period of 12-22 days after wounding,the percentage of S phase cells increased and reached its peak value on the 22nd day.When most of cells were in S phase and arrested dramatically.Through the whole healing process,the percentage of G2/M in wound of the irradiation side was lower than that of the non irradiated wound.The BrdU positive cells were fibroblasts,endothelial cells and smooth muscle cells.Conclusion These results suggest that G1 block,S phase arrest,and switch of G2/M with suppression of mitotic activity of these cells are induced by local 5.21 Gy soft X ray irradiation.Therefore,wound healing delay is induced partly by cell cycle arrest. |
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