| SHU Chongxiang,LOU Shufen,CHENG Tianmin.Effects of γ irradiation on rate of wound healing in a scored confluent monolayer of cells and the repair-promoting role of W11-a12[J].Chinese Journal of Radiological Medicine and Protection,2002,22(3):173-175 |
| Effects of γ irradiation on rate of wound healing in a scored confluent monolayer of cells and the repair-promoting role of W11-a12 |
| Received:November 19, 2001 |
| DOI： |
| KeyWords:Radiation injury Wound healing Fibroblast Vascular endothelial cells W11-a12 |
| FundProject:国家重点基础研究发展规划“973”专项经费资助项目(G1999054205);重庆市科委院士基金资助项目(985081) |
| Author Name | Affiliation | | SHU Chongxiang | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | LOU Shufen | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | CHENG Tianmin | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 李树楠 | 云南大理医学院药理学教研室 | | 冉新泽 | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China |
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| Abstract:: |
| Objective To investigate the effects of ionizing radiation on healing rate of experimental wound in a scored confluent monolayer of fibroblasts and vascular endothelial cells and the repair promoting effect of W11-a12.Methods The healing rates of the experimental wound in a scored confluent monolayer of 3T3 cells or ECV304 cells irradiated with 6 Gy 60 Co gamma rays were assayed by measuring the width of the wound.Results After irradiation,the closure of scored wounds both in a confluent monolayer of 3T3 cells and in that of ECV304 cells was significantly delayed.The scored wound in a confluent monolayer of 3T3 cells was completely closed in the sham irradiation group,but it was only 77% in the irradiation group at the tenth hour post wounding.The healing rate of the scored wound in a confluent monolayer of irradiated ECV304 cells was 83.6% of that in the sham irradiation group W11-a12 had good promoting action on the closure of wounds in scored confluent monolayers of these two kinds of cells.Conclusion The direct inhibitory effects of irradiation on the proliferating and migrating capacity of both fibroblasts and vascular endothelial cells might be one of the important reasons for the delay of healing in irradiation impaired wounds and W11-a12 could promote healing of irradiation impaired wound by means of enhancing cell migratation and proliferation directly. |
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