| SHU Chongxiang,CHENG Tianmin,LI Shunan.Inhibitory effect of 5 Gy total-body irradiation and promoting role of W11-a12 on wound fibroblast function in rats[J].Chinese Journal of Radiological Medicine and Protection,2002,22(3):170-172 |
| Inhibitory effect of 5 Gy total-body irradiation and promoting role of W11-a12 on wound fibroblast function in rats |
| Received:November 19, 2001 |
| DOI： |
| KeyWords:Radiation injury Wound healing Fibroblasts Fibronectin Growth factor |
| FundProject:国家重点基础研究发展规划“973”专项经费资助项目(G1999054205);重庆市科委院士基金资助项目(985081) |
| Author Name | Affiliation | | SHU Chongxiang | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038 | | CHENG Tianmin | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038 | | LI Shunan | Department of Pharmacology, Dali Medical College, Yunnan 671000, China | | 冉新泽 | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038 |
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| Abstract:: |
| Objective To investigate the inhibitory effects of 5 Gy whole body irradiation on wound fibroblast function and the promoting role of W11-a12 on these cells.Methods Two full thickness skin incision wounds,in which polyvinyl alcohol sponges were planted,were inflicted on both sides of the back of rats after they had received 5 Gy gamma ray whole body irradiation.The percentages of immunocytochemistry positive fibroblasts for fibronectin (FN),transforming growth factor β1(TGFβ1) and basic fibroblasts growth factor (bFGF) in the wound were observed.Results The percentages of immunocytochemistry positive fibroblasts for FN,TGFβ1 and bFGF in wound were all significantly reduced on day 3 and 5 after 5 Gy whole body irradiation,but the degree of reduction varied in severity (bFGF>FN>TGFβ1).W11-a12 could increase the percentages of immunopositive cells for FN,TGFβ1 and bFGF both in simple wound and in wound impaired by irradiation.Conclusion Whole body irradiation injury could cause significant inhibitory effect on wound fibroblast function of synthesizing and secreting FN,TGFβ1 and bFGF.W11-a12 could promote irradiation impaired wound healing by means of enhancing these functions of wound fibroblasts. |
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