| QU Jifu,CHENG Tianmin,XU Linshui.Apoptosis of dermal fibroblasts in wounds combined with radiation injury and its mechanisms[J].Chinese Journal of Radiological Medicine and Protection,2002,22(3):155-158 |
| Apoptosis of dermal fibroblasts in wounds combined with radiation injury and its mechanisms |
| Received:November 19, 2001 |
| DOI： |
| KeyWords:Radiation Wound Fibroblast Apoptosis Bcl-2 Bax |
| FundProject:国家重点基础研究发展规划“973”专项经费资助项目(G1999054205) |
| Author Name | Affiliation | | QU Jifu | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | CHENG Tianmin | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | XU Linshui | 生化教研室 | | 史春梦 | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 林远 | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 阎国和 | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 冉新泽 | Institute of Combined Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China |
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| Abstract:: |
| Objective To study apoptosis of dermal fibroblasts in wounds combined with total body irr adiaation (TBI) injury and its mechanisms.Methods We determined the amount of dermal fibroblasts in wounds of rats using histological method,observed apoptosis of dermal fibroblasts in wounds by transmission electron microscope and TUNEL assay,and detected the expression of Bcl-2 and Bax in wounds with immunohistochemistry method.Results The ratio of fibroblastic apoptosis in wounds combined with TBI injury significantly increased as compared with that of simple incision injury.The contents of Bax in wounds combined with TBI injury were significantly higher than those of simple incision injury,and the contents of Bcl-2 and Bcl-2/Bax ratio in the fomer were significantly lower.Conclusion Apoptosis of dermal fibroblasts in wounds combined with TBI injury increases significantly,which causes reduction of the amount of fibroblasts.Abnormalities of Bcl-2,Bax and Bcl-2/Bax ratio are important reasons of the increase of fibroblastic apoptosis. |
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