| DU Nan,PEI Xuetao,LUO Chengji.Effect of expression of hematopoietic growth factors regulated by radio-inducible promoter on radiation injury of bone marrow stromal cells[J].Chinese Journal of Radiological Medicine and Protection,2002,22(2):80-82 |
| Effect of expression of hematopoietic growth factors regulated by radio-inducible promoter on radiation injury of bone marrow stromal cells |
| Received:January 15, 2001 |
| DOI： |
| KeyWords:Radiation-inducible promoter Hematopoietic growth factor stromal cells |
| FundProject:国家自然科学基金资助项目(39900040);国家杰出青年自然科学基金资助项目(39825111) |
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| Abstract:: |
| Objective To explore radioresistance -improving and radio-protective effects of hematopoietic growth factor genes transfected into HFCL stromal cells.Methods Radioresistance of gene-transfected cells to radiation was detected by dose survival curve and 3H-TdR incorporation rate,Colony forming assay on cultured stroma layer was examined.Results The gene-modified cells (HFCL/EFG and HFCL/FG) showed more resistant to ionizing radiation than control HFCL cells by dose survival curve, 3H-TdR incorporation rate.The transfected genes had effects of protecting hematopoiesis and radioprotection by colony forming assay.ConclusionStromal cells transduced with hematopoietic growth factor genes can improve radioprotective effects. |
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