| ZHANG Hao,MAO Bingzhi,Dong Bo.Stimulating recovery of hemopoietic and immunological functions by cotransferring IL-6 and SCF genes in irradiated mice[J].Chinese Journal of Radiological Medicine and Protection,2002,22(2):77-79 |
| Stimulating recovery of hemopoietic and immunological functions by cotransferring IL-6 and SCF genes in irradiated mice |
| Received:August 10, 2008 |
| DOI： |
| KeyWords:QXMSC IL-6 SCF |
| FundProject:国家自然科学基金资助项目(39870716) |
| Author Name | Affiliation | | ZHANG Hao | Beijing Institute of Radiation Medicine, Acadamic Military of Medicine, Bejing 100850, China | | MAO Bingzhi | Beijing Institute of Radiation Medicine, Acadamic Military of Medicine, Bejing 100850, China | | Dong Bo | Beijing Institute of Radiation Medicine, Acadamic Military of Medicine, Bejing 100850, China | | 陈肖华 | Beijing Institute of Radiation Medicine, Acadamic Military of Medicine, Bejing 100850, China | | 张军权 | Beijing Institute of Radiation Medicine, Acadamic Military of Medicine, Bejing 100850, China | | 罗庆良 | Beijing Institute of Radiation Medicine, Acadamic Military of Medicine, Bejing 100850, China | | 秦凤华 | Beijing Institute of Radiation Medicine, Acadamic Military of Medicine, Bejing 100850, China |
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| Abstract:: |
| Objective To explore the hemopoietic and immunological functions of retroviral-mediated IL-6 and SCF genes cotransferred into bone marrow stromal cells in irradiated mice.Methods IL-6 and SCF cDNA were recombined with retroviral vector pLXSN by gene recombination technolgy.Number of peripheral blood and bone marrow cells,CFU-GM of bone marrow cells,LTT and CD4/CD8 ratio of spleen were also assayed in vivo.Results The bone marrow stromal cells transferred with IL- 6 and SCF genes could stimulate the recovery of hemopoietic and immunological functions in irradiated mice in vivo.ConclusionIL- 6 and SCF genes are simultaneously expressed in bone marrow stromal cells,which provide a basis for studies on hematopoietic regulation by gene-transfected bone marrow stromal cells. |
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