ZHOU Pingkun,SUI Jianli.cDNA cloning and transcriptional controlling of a novel low dose radiation-induced gene and its function analysis.[J].Chinese Journal of Radiological Medicine and Protection,2002,22(2):73-76 |
cDNA cloning and transcriptional controlling of a novel low dose radiation-induced gene and its function analysis. |
Received:September 10, 2001 |
DOI: |
KeyWords:Radiation-induced gene Transcriptional control Low dose radiation Helicase Bioinformatics |
FundProject:国家自然科学基金资助项目(39870214);“十五”军队医学杰出中青年基金资助项目(01J006);国家高技术研究发展计划(863)资助项目(2001AA221271) |
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Abstract:: |
Objective To clone a novel low dose radiation-induced gene (LRIGx) and study its function as well as its transcriptional changes after irradiation.Methods Its cDNA was obtained by DDRT-PCR and RACE techniques.Northern blot hybridization was used to investigate the gene transcription.Bioinformatics was employed to analysis structure and function of this gene.Results LRIGx cDNA was cloned.The sequence of LRIGx was identical to a DNA clone located in human chromosome 20 q 11.2-12.Bioinformatics analysis predicted an encoded protein with a conserved helicase domain.Northern analysis revealed a~8.5 kb transcript which was induced after 0.2 Gy as well as 0.02 Gy irradiation,and the transcript level was increased 5 times at 4 h after 0.2 Gy irradiation.The induced level of LRIGx transcript by 2.0 Gy high dose was lower than by 0.2 Gy.ConclusionA novel low dose radiation-induced gene has been cloned.It encodes a protein with a conserved helicase domain that could involve in DNA metabolism in the cellular process of radiation response. |
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