| .Effects of introducing wild-type p53 gene on the radiosensitivity of SKOV-3 ovarian cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2002,22(1):29 |
| Effects of introducing wild-type p53 gene on the radiosensitivity of SKOV-3 ovarian cancer cells |
| Received:September 04, 2001 |
| DOI： |
| KeyWords:Ovarian tumor Gene p53 Transfection Radiosensitivity |
| FundProject:辽宁省自然科学基金资助项目(972252) |
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| Abstract:: |
| Objective To study the effect of wild-type p53 gene on the radiosensitivity of SKOV-3 ovarian cancer cells. Methods Recombinant eukaryotic expression vector pcDNA3 containing full-length human wild-type p53 cDNA was introduced by lipofectamine-mediated gene transfection into cultured SKOV-3 cells which had been irradiated with 2 and 4 Gy X-rays, respectively.The radiosensitivities of the tumor cells with different p53 status were studied. Results The number of colonies in the SKOV-3, SKOV-3-vect, and SKOV-3-p53 groups decreased by 18.6%, 22.9% and 44.5%, respectively with 2 Gy irradiation, and decreased by 63.6%, 64.9% and 88.9%, respectively with 4 Gy irradiation.After introduction of p53 cDNA, the cell number in S phase and the ratio of G2/M phase of tumor cells decreased and the ratio of G1/G-0 phase increased.The introduction of p53 gene into cells led to cell cycle arrest in G1 phase. Conclusion Exogenous introduction of wild-type p53 cDNA into SKOV-3 ovarian cancer cells can increase their radiosensitivity. |
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