| LIU Xiaodong,LIU Shuzheng,MA Shumei.Changes of IL-10 expression in splenocytes after X-irradiation of different doses[J].Chinese Journal of Radiological Medicine and Protection,2002,22(1):10-12 |
| Changes of IL-10 expression in splenocytes after X-irradiation of different doses |
| Received:March 25, 2001 |
| DOI： |
| KeyWords:Ionizing irradiation Splenic lymphocytes IL-10 LDR HDR |
| FundProject:国家自然科学基金资助项目(39570188) |
| Author Name | Affiliation | | LIU Xiaodong | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | LIU Shuzheng | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | MA Shumei | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | 刘杨 | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China |
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| Abstract:: |
| Objective To observe the time course changes in the expression of IL-10 in splenocytes after whole body irradiation (WBI) with different doses of X-rays. Methods Northern blot and flow cytometry(FCM) were adopted to detect the changes of IL-10 at mRNA and protein levels respectively. Results FCM data indicated IL-10 protein synthesis decreased beginning from 2 h after exposure and remained at low level up to 48 h after 75 mGy irradiation.The increase of IL-10 synthesis was detectable after 2 Gy irradiation.By northern blot we found IL-10 mRNA decreased significantly after WBI with 75 mGy X-rays(low dose radiation,LDR),while it increased at 2 h after WBI with 2 Gy X-rays (high dose radiation,HDR) . Conclusion The results suggest LDR and HDR have different effects on IL-10. |
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