| HE Shujie,YE Fei,LIU Shuzheng.Relationship between X-rays-induced activation of transcription factors and apoptosis[J].Chinese Journal of Radiological Medicine and Protection,2002,22(1):4-6 |
| Relationship between X-rays-induced activation of transcription factors and apoptosis |
| Received:March 21, 2001 |
| DOI： |
| KeyWords:X-rays Thymocytes Apoptosis Transcription factors NF-κB CREB OCT-1 |
| FundProject:国家自然科学基金资助项目(39570188) |
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| Abstract:: |
| Objective To investigate the role of activation of transcription factors in the induction of apo- ptosis of immune cells after whole-body irradiation (WBI)with X-rays by comparing the time course of the changes of DNA-binding activity of several transcription factors and that of apoptosis in mouse thymocyte after 2 Gy X-rays. Methods The dynamic changes of the activity of transcription factors NF-κB,CREB and OCT-1,as well as thymocyte apoptosis after whole body irradiation with 2 Gy X-rays were respectively examined with electrophoresis mobility shift assay (EMSA) and flow cytomentry (FCM). Results There was a significant distinction between the changes of DNA-binding activity of two dimers of NF-κB(p50/p50 and p50/p65) in thymocytes after WBI with 2 Gy X-rays,with significant increase in the binding activity of NF-κB homodimer p50/p50.In addition,the activity of CREB and OCT-1 also increased significantly.Their activity peaks at 4-12 h after WBI coincided well with the peaking time of thymocyte apoptosis. Conclusions WBI with 2 Gy X-rays mainly induces the activation of NF-κB homodimer p50/p50.The results suggest that the three transcription factors including NF-κB p50/p50,CREB and OCT-1 might act synergistically in the induction of apoptosis in thymocytes after WBI with 2 Gy X-rays. |
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