| DU Nan,PEI Xuetao,LUO Chengji.Radioprotective effect of hematopoietic growth factor gene therapy regulated by Egr-1 promoter on radiation injury of SCID mice[J].Chinese Journal of Radiological Medicine and Protection,2002,22(1):1-3 |
| Radioprotective effect of hematopoietic growth factor gene therapy regulated by Egr-1 promoter on radiation injury of SCID mice |
| Received:March 04, 2001 |
| DOI： |
| KeyWords:Egr-1 Promoter Hematopoietic growth factor Gene therapy Radioprotective effect |
| FundProject:国家自然科学基金资助项目(39900040);国家杰出青年自然科学基金资助项目(39825111) |
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| Abstract:: |
| Objective To explore the radioprotective effect of the expression of hematopoietic growth factors regulated by radio-inducible promoter on radiation injury. Methods The human FL cDNA and EGFP cDNA were linked together with an internal ribosome entry site(IRES) and then inserted into the eukaryotic expression vector pCI-neo with the Egr-1 promoter(Egr-EF),and further transduced into bone marrow stromal cell lines HFCL (HFCL/EF).The HFCL/EF and CD34 + cells from human umbilical cord blood were transplanted i.v. one after the other into sublethally irradiated severe combined immunodeficient (SCID) mice.The number of peripheral blood WBC and human cells engrafted in recipent mice were detected by flow cytometry and CFU-GM assay. Results In contrast to two control groups (HFCL and HFCL/F),HFCL/EF (the Egr-1 regulatory element-drived expression of FL gene therapy) resulted in a proportionally obvious increase in the number of the WBC at early stage after irradiation.Significant diifferences were found for CD45 +,CD 34+,CFU-GM,and nucleared cells in the bone marrow. Conclusion Hematopoietic growth factor gene therapy regulated by radio-inducible promoter has radioprotective effect on radiation hematopoietic injury. |
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