| ZHU Jiankun,ZHANG Xueguang,XU Zhixiang.Radioprotective effects of recombinant human Flt3 ligand and other cytokines on immunocytes in vitro[J].Chinese Journal of Radiological Medicine and Protection,2001,21(6):424-427 |
| Radioprotective effects of recombinant human Flt3 ligand and other cytokines on immunocytes in vitro |
| Received:November 21, 2000 |
| DOI： |
| KeyWords:Cytokines Flt3 ligand Immune cells Irradiation Apoptosis |
| FundProject:国防科工委国防预研基金资助项目(Y5573162);国际原子能机构(IAEA)基金资助项目(CRP/9/025) |
| Author Name | Affiliation | | ZHU Jiankun | Department of Immunology, Medical Biotechnology Institute, School of Life Science, Suzhou University, Suzhou 215007, China | | ZHANG Xueguang | Department of Immunology, Medical Biotechnology Institute, School of Life Science, Suzhou University, Suzhou 215007, China | | XU Zhixiang | Department of Immunology, Medical Biotechnology Institute, School of Life Science, Suzhou University, Suzhou 215007, China | | 徐颖 | Department of Immunology, Medical Biotechnology Institute, School of Life Science, Suzhou University, Suzhou 215007, China | | 赵仁亮 | 青岛医学院附属医院 | | 於葛华 | Department of Immunology, Medical Biotechnology Institute, School of Life Science, Suzhou University, Suzhou 215007, China | | 强亦忠 | Department of Immunology, Medical Biotechnology Institute, School of Life Science, Suzhou University, Suzhou 215007, China |
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| Abstract:: |
| Objective To investigate the immunoprotective potential of recombinant human Flt3 ligand (rhFL) and other cytokines on irradiated peripheral blood mononuclear cells (PBMC) in vitro . Methods The phenotypes of PBMCwere analysed by flow cytometric analysis.The proliferative-promoting effect of rhFL on irradiated PBMC was measured by 3H-thymidine incorporation assay.Dendritic cells (DC) were induced from irradiated PBMC by a cytokine cocktail with or without rhFL.Apoptotic rate of irradiated PBMC was measured with PI-Annexin Vand DNAladder. Results CD8+ and CD56+ cells in PBMC decreased significantly at 24 h and 48 h post-irradiation.If the PBMC were cultured with rhFL before irradiation and with IL-2 after irradiation CD8+,CD56+ cells increased and approached to the non-irradiated level.The number of DC induced from irradiated PBMC increased by the addition of rhFL.rhFL stimulated the proliferation of the PBMC,but its effect was weaker than that of PHA.Irradiation caused a significant increase of apoptosis in PBMC.Addition of rhFL to the culture reduced apoptosis of PBMC significantly. Conclusion rhFLprotects the immunocytes in 15 Gy γ-irradiation treated PBMC from apoposis,so it shows promising irradiation-protective effects on immune system. |
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