| QIANG Yonggang,LIAO Yonghua,ZOU Rongzhu.The interfering effect of persistent low dose rate irradiation on tumor formation in mice by chemical carcinogen[J].Chinese Journal of Radiological Medicine and Protection,2001,21(6):421-423 |
| The interfering effect of persistent low dose rate irradiation on tumor formation in mice by chemical carcinogen |
| Received:September 04, 2000 |
| DOI： |
| KeyWords:Chemical carcinogen Lung tumor γ irradiation p53 |
| FundProject:广东省医药卫生科研基金资助项目(1997237) |
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| Abstract:: |
| Objective This paper studies the interfering effect of persistent low dose rate irradiation of γ-rays on the formation of lung tumors in mice of two generations and on the regulation of the proliferation of tumor cells as well as p53 gene under the effect of ethyl carbamare,a chemical carcinogenic substance. Methods Kunming mice were subjected to irradiation of 1.20 Gy 137 Cs γ rays(dose rate:20 μGy/min) and the single abdominal subcutaneous injection dose of chemical carcinogentic substance was 2000 mg/kg.The formation of lung tumor cells was observed and lung and lung tumor tissue PCNA and p53 anti-cancer gene were analysed by means of immunohistochemical method of biotin marker. Results Persistent low dose irradiation did not markedly interfere with the effect of chemical carcinogenic substances on formation of lung tumor and the incidence of lung tumor of mice in the experimental and control group was 94% and 95.83%,respectively,with no significant difference (χ2=0004, P >0.05).The incidence of tumor in the offspring mice,whose sterilization,pregnancy and growth were all under persistent low dose rate irradiation,was 56.6%,being evidently lower than that of parental mice (χ2=20.81, P <0.01).In addition,the diameter of tumor and the average number of tumors in offspring mice was also smaller than those of parental mice,the mutation of p53 in offspring mice and the expression of PCANwere greatly reduced. Conclusion Persistent low dose rate irradiation can induce an "adaptive reaction" of offspring mice to lung tumor for mation and reduce the proliferation of tumor cells in offspring mice. |
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