| DENG Yubin,LI Shunong,ZHOU Pingkun,et al.Studies on hHR21sp expression induced by irradiation in the peripheral blood cells and human cell line (CEM)[J].Chinese Journal of Radiological Medicine and Protection,2001,21(6):413-416 |
| Studies on hHR21sp expression induced by irradiation in the peripheral blood cells and human cell line (CEM) |
| Received:April 17, 2001 |
| DOI： |
| KeyWords:Ionizing radiation(IR) Gene expression Gene hHR21 |
| FundProject:CMB(美国中华医学基金会)基金(96630);2000年教育部回国人员启动基金资助项目(2000年度) |
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| Abstract:: |
| Objective To study the effects of UV and gamma-ray irradiation on hHR21sp transcription in a human T lymphocytic leukemia cell line,CEM. Methods Total RNA was extracted from normal human peripheral blood mononuclear cells (PB-MNC) and CEM cells after exposure to UV and gamma-ray irradiation.The level of hHR21 transcripts was deteced using RT-PCR with southern blot hybridization and phosphorlmage autoradiogram analysis. Results hH21sp mRNAincreased significantly at 3 h to 9 h after UVirradiation in PB-MNC and CEM cells,and the induction doses were 40 to 80 J/Mand 80-120 J/Mfor PB-MNC and CEM cells,respectively.In PB-MNC,hHR21sp mRNA increased at 6 to 9 h after 1 to 5 Gy of gamma-ray irradiation.In contrast,hHR21sp mRNA was induced only at 3 h after irradiation in CEM cells,and its peak appeared at 9 h after irradiation with 3 Gy. Conclusion The results revealed that hHR21sp gene transcription could be induced by UVas well as gamma-ray irradiation.The levles of hHR21sp mRNA induced by gamma-ray irradiation was obviously different between PB-MNC and CEM cells. |
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