| LUO Ying,SUI Jianli,TIE Yi,et al.Cloning of low dose radiation induced gene RIG1 by RACE based on non-cloned cDNA library[J].Chinese Journal of Radiological Medicine and Protection,2001,21(6):407-409 |
| Cloning of low dose radiation induced gene RIG1 by RACE based on non-cloned cDNA library |
| Received:September 18, 2000 |
| DOI： |
| KeyWords:Non-cloned cDNA library Enhanced RACE PCR Probe hybridization RIG1 gene |
| FundProject:国家自然科学基金资助项目(39870214) |
| Author Name | Affiliation | | LUO Ying | Institute of Radiation Medicine, Beijing 100850, China | | SUI Jianli | Institute of Radiation Medicine, Beijing 100850, China | | TIE Yi | Institute of Radiation Medicine, Beijing 100850, China | | 张元平 | 兰州军区总医院临床试验科 | | 周平坤 | Institute of Radiation Medicine, Beijing 100850, China | | 孙志贤 | Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract:: |
| Objective To obtain full-length cDNA of radiation induced new gene RIG1 based on its ESTfragment. Methods Based on non-cloned cDNA library,enhanced nested RACE-PCR and biotin-avidin labeled probe for magnetic bead purification was used to obtain full-length cDNA of RIG1. Results About 1 kb of 3' end of RIG1 gene was successfully cloned by this set of methods and cloning of RIG1 5' end is proceeding well. Conclusion The result is consistent with the design of experiment.This set of protocol is useful for cloning of fu11-lengh gene based on ESTfragment. |
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