| YANG Guang,PEI Xuetao,LI Liang,et al.Regulation of megakaryocytopoiesis by TPO gene-modified stromal cells[J].Chinese Journal of Radiological Medicine and Protection,2001,21(5):335-338 |
| Regulation of megakaryocytopoiesis by TPO gene-modified stromal cells |
| Received:November 10, 2000 |
| DOI： |
| KeyWords:Hematopoietic cells Megakaryocytic cells Stromal cells Thrombopoietin(TPO) |
| FundProject:国家重点基础研究发展规划项目(973)(G1999053903);国家杰出青年科学基金资助项目(39825111) |
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| Abstract:: |
| Objective To study the effect of TPOgene-modified stromal cells on stimulating megakaryocytopoiesis. Methods Retroviral vectors carrying TPOgene were constructed and used to transfect the stromal cell line HFCL by using a "Ping-pang" technique.The expression of TPOgene was detected by Northern blot and dependent cell line TD-3.By contrast with expansions supported by non-modified stromal cells,CD34+ hematopoietic cells from cord blood were expanded on gene-modified stromal cells for 7 days.Finally,percentages of CD34 +CD41+ cells and CD41+CD61+ cells were analyzed by flow cytometry. Results The stromal cell line HFCLcould express the TPOgene transduced by using the retroviral vectors,and the activity of TPO in supernatant of the tranduced HFCLcells was 075 ng/ml.The percentages of CD34+CD41+ megakaryocyte-committed progenitors in the presence of TPO gene-modified HFCL cells(137%±20%) were higher than those in the nontransduced HFCL cells (65%±18%),and there was no difference statistically on the percentages of the CD41+CD61+ cells between the cultures supported by transduced and non-tranduced stromal cells. Conclusion Stromal cell line HFCL could express the TPOwith biological activities after transfected by retroviral vectors,and enhance the growth of CD34+CD41+ megakaryocyte-committed progenitor cells. |
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