| WU Congmei,LI Xiuyi,LIU Shuzheng.Construction of pEgr.p-TNFα and its expression in NIH3T3 cells induced by ionizing irradiation[J].Chinese Journal of Radiological Medicine and Protection,2001,21(5):332-334 |
| Construction of pEgr.p-TNFα and its expression in NIH3T3 cells induced by ionizing irradiation |
| Received:December 10, 2000 |
| DOI： |
| KeyWords:Egr-1 promoter X-ray induce-TNF α expression level Low dose irradiation |
| FundProject:国家自然科学基金资助项目(39970229) |
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| Abstract:: |
| Objective To isolate and amplify Egr-1 promoter,construct pEgr.p-TNFα and study its response to different doses of ionizing radiation. Methods Egr-1 promote was isolated from genomic DNAby PCR to construct pEgr.p-TNFα expression plasmid.Plasmids were transfected into NIH3T3 cells with lipsome and the expression level of TNFα was detected by ELISAafter irradiation with different doses of X-ray . Results The sequence of Egr-1 promoter obtained was essentially same as reported.Egr-1 promoter and TNF α cDNA was inserted into expression vector correctly.Eight hours after irradiation with different doses of X-rays,the expression level of TNFα was higher than that of nonirradiated group( P <005～0001). Conclusion Egr-1 promoter obtained can be activated by ionizing irradiation and regulate the expression of downstream gene.Low dose irradiation is for the first time found to be able to induce the expression of the downstream gene.The observation may be of potential significance in tumor therapy. |
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