| ZHANG Xin,XU Shiguo,ZHANG Yuheng,et al.Radiobiological effects of soft X-ray with resonance energy of carbon K-absorption edge from synchrotron radiation on plasmid pBK-CMV DNA[J].Chinese Journal of Radiological Medicine and Protection,2001,21(5):321-324 |
| Radiobiological effects of soft X-ray with resonance energy of carbon K-absorption edge from synchrotron radiation on plasmid pBK-CMV DNA |
| Received:December 20, 2000 |
| DOI： |
| KeyWords:Synchrotron radiation Soft X-ray Biological effectiveness |
| FundProject:国家自然科学基金资助项目(39770195) |
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| Abstract:: |
| Objective To study the radiation effects of synchrotron-produced 0.28 keVcarbon Ksoft X-ray on the plasmid pBK-CMV DNA with 60Co γ ray as reference radiation. Methods By using a special designed sample chamber,plasmid pBK-CMV DNA was exposured to Ck soft X-ray in water solution,and then transformed into the E.coli XL1-Blue.The changes of average biological survival rates and mutation frequency were in vestigated as the endpoints to study the radiation effects of Ck soft X-ray on the plasmid pBK-CMV DNA. Results Compared with 60Co-γ ray,Ck soft X-ray decrease the average biological survival rates of plasmid pBK-CMV DNAby four times and increase the mutation frequency by 1.5 times-2 times. Conclusion the biological effects of Ck soft X-ray is more effective than that of 60Co-γ ray. |
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