| XU Zhixiang,ZHANG Xueguang,QIANG Yizhong,et al.The influence of Flt3 ligand on proliferation of irradiated microvascular endothelial cells[J].Chinese Journal of Radiological Medicine and Protection,2001,21(4):286-289 |
| The influence of Flt3 ligand on proliferation of irradiated microvascular endothelial cells |
| Received:November 21, 2000 |
| DOI： |
| KeyWords:Flt3 ligand Hematopoietic micro-environment Microvascular endothelial cell(ECVs) Irradiation Proliferation Apoptosis |
| FundProject:国防科工委国防预研基金资助项目(Y5573162);国际原子能机构(IAEA)基金资助项目(CRP9025) |
| Author Name | Affiliation | | XU Zhixiang | Department of Immunology, Medical Biotechnology Institute, School of Life Science, Soochow University, Suzhou 215007, China | | ZHANG Xueguang | Department of Immunology, Medical Biotechnology Institute, School of Life Science, Soochow University, Suzhou 215007, China | | QIANG Yizhong | Department of Immunology, Medical Biotechnology Institute, School of Life Science, Soochow University, Suzhou 215007, China | | 徐颖 | Department of Immunology, Medical Biotechnology Institute, School of Life Science, Soochow University, Suzhou 215007, China | | 施勤 | Department of Immunology, Medical Biotechnology Institute, School of Life Science, Soochow University, Suzhou 215007, China | | 朱剑昆 | Department of Immunology, Medical Biotechnology Institute, School of Life Science, Soochow University, Suzhou 215007, China | | 丁天 | 上海复旦大学生化系 |
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| Abstract:: |
| Objective To investigate the influence of FL on proliferation of irradiated microvascular endothelial cells (ECVs),and possible mechanism of FL in radiation protection of ECVs. Methods The ECVs were screened for Flt3 receptor expression by flow cytometric analysis.The proliferation of ECVs stimulated by FL was measured by the microculture tetrazolium assay (MTT).The apoptosis of ECVs caused by irradiation was measured with Annexin V PI.Two apoptosis related genes,Bcl-XL and Bax,were also analyzed by RT-PCR. Results Flt3 receptors were expressed on the surface of ECVs.FL stimulated the proliferation of ECVs at very low concentrations (0.5-15 ng/ml) with the maximum stimulation at 15 ng/ml.A significant increase in Bax activity and a decrease in Bcl XL activity were seen at 24 h and 48 h post-irradiation,respectivety. When the culture medium with FL was added 2 h before or immediately after irradiation,the expression of Bax fell sharply at 24 h and 48 h post-irradiation.The change in Bcl-XL activity was not so marked and a mild increase in Bcl-XL expression was seen only at 48 h post-irradiation.FL inhibited the apoptosis of ECVs caused by irradiation and stimulated the proliferation of irradiated ECVs. Conclusion FL down-regulates the expression of Bax in irradiated ECVs,and inhibits the apoptosis of the ECVs.Thus,FL may find a use in radio-protection of hematopoietic cells via protection of the microvascular endothelial cells in the bone marrow microenvironment. |
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