| SHI Mei,WEI Lichun,SUN Chaoyang.Influence of radiotherapy on expression of the proliferating cell nuclear antigen(PCNA) and c-fos in human cervical cancer[J].Chinese Journal of Radiological Medicine and Protection,2001,21(4):273-275 |
| Influence of radiotherapy on expression of the proliferating cell nuclear antigen(PCNA) and c-fos in human cervical cancer |
| Received:December 05, 2000 |
| DOI： |
| KeyWords:Proliferating cell nuclear antigen c-fos Human cervical cancer Radiotherapy |
| FundProject:国家自然科学基金资助项目(30040012) |
| Author Name | Affiliation | | SHI Mei | Department of Radiatherapy, Xijing Hospital
Fourth Military Medical University, Xi'an 710032, China | | WEI Lichun | Department of Radiatherapy, Xijing Hospital
Fourth Military Medical University, Xi'an 710032, China | | SUN Chaoyang | Department of Radiatherapy, Xijing Hospital
Fourth Military Medical University, Xi'an 710032, China | | 马海昕 | Department of Radiatherapy, Xijing Hospital
Fourth Military Medical University, Xi'an 710032, China | | 郭燕 | Department of Radiatherapy, Xijing Hospital
Fourth Military Medical University, Xi'an 710032, China |
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| Abstract:: |
| Objective To investigate changes of proliferating cell nuclear antigen(PCNA)expression in human cervical cancer following irradiation. Methods Immunohistochemical staining for PCNA was performed in frozen sections of formalin-fixed cervical cancer biopsy tissues. Results The majority of the cancer cells showed PCNA-immunoreactivity before irradiation.Following irradiation(30-40 Gy/15-20 f)PCNA-immunopositive staining was hardly detectable in most of the cancer cells.The PCNA-immunoreactivity,however, increasd after radiotherapy and moderate or heavy immunopositive staining for PCNA was seen in irradiated mesenchymal tissue cells. On the other hand after irradiation Fos-immunoreactivity decreased remarkably and Fos-immunoposi-five staining was hardly detectable in most of cancer cells. No obvious change in Fos-immunoreactivity however was seen in mesenchymal connective tissue following irradiation. Conclusion Irradiation inhibits PCNA and c-fos expression in ceivicval cancer cells whereas it induces the expression of PCNA in mesenchymal tissue cells The present results suggest that expression of PCNA and c-fos may be regarded as a molecular marker for evaluat-ing the cancer cell proliferation and mesenchymal tissue repair during radiotherapy of human cervical cancer. |
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