| WU Congmei,LI Xiuyi,LIU Shuzheng.Experimentalal study on anti-tumor effect of pcEgr-IFNγ gene-radiotherapy[J].Chinese Journal of Radiological Medicine and Protection,2001,21(4):236-238 |
| Experimentalal study on anti-tumor effect of pcEgr-IFNγ gene-radiotherapy |
| Received:April 30, 2001 |
| DOI： |
| KeyWords:pcEgr-IFNγ reconstruction plasmid X-ray irradiation B16 melanoma Gene-radiotherapy |
| FundProject:国家自然科学基金资助项目(39970229) |
| Author Name | Affiliation | | WU Congmei | MH Radiobiology Research Unit, School of Preventive Medicine, Jilin University, Changchun 130021, China | | LI Xiuyi | MH Radiobiology Research Unit, School of Preventive Medicine, Jilin University, Changchun 130021, China | | LIU Shuzheng | MH Radiobiology Research Unit, School of Preventive Medicine, Jilin University, Changchun 130021, China |
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| Abstract:: |
| Objective To study the anti-tumor effect of IFN-γ gene-radiotherapy to murine melanoma and its immunologic mechanism. Methods pcEgr-IFNγ plasmids were injected locally into tumor,and 36 hours later,the tumors were given 20 Gy X-ray irradiation.Tumor growth at different time,IFN-γ expression 3 days later and immunologic indexes 15 days later were detected. Results At 3-15 days after pcEgr-IFNγ gene-radiotherapy,the tumor growth rate was lower than that of irradiation alone group.It was also lower than that of gene thempy alone group and control plasmid combined with X-ray irradiation group significantly. Day 3 tumor IFN y expression was higher than that of plasmid treatment alone group. NK activity, IL-2 and IFN y secretion activities were higher than those of genetherapy alone and irradiation alone groups significantly. Conclusion The anti-tu-mor effect of IFN y gene-radiotherapy is better than that of either of them applied solely. Its mechanism might be conccerned with the higher expression of IFN y induced by irradiation in tumors and activation of anti-tumor immu-nologic functions. |
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