| ZHUANG Yongzhi,WANG Junjie,ZHANG Zhanchum.The mechanism of inhibitory effect of γ-ray irradiation on rat vascular smooth muscle cells[J].Chinese Journal of Radiological Medicine and Protection,2001,21(4):233-235 |
| The mechanism of inhibitory effect of γ-ray irradiation on rat vascular smooth muscle cells |
| Received:April 30, 2001 |
| DOI： |
| KeyWords:VSMCs γ-ray irradiation apopotosis restenosis |
| FundProject:国家自然科学基金资助项目(39770192) |
| Author Name | Affiliation | | ZHUANG Yongzhi | Department of Oncology, the Third Hospital of Peking University, Beijing 100083, China | | WANG Junjie | Department of Oncology, the Third Hospital of Peking University, Beijing 100083, China | | ZHANG Zhanchum | Department of Oncology, the Third Hospital of Peking University, Beijing 100083, China | | 贾廷珍 | Department of Oncology, the Third Hospital of Peking University, Beijing 100083, China |
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| Abstract:: |
| Objective To investigate the inhibitory effect of γ-ray irradiation on rat vascular smooth muscle cells (VSMCs). Methods Dose-survival curve of VSMCs was figured by colony formation.The effect of γ-ray irradiation on viability and proliferation of VSMCs was observed by 3H incorporation.Flow cytometry and DNA Ladder were used to detect the apopotogenic effect of γ-ray irradiation on VSMCs. Results The values of D0,Dq,D37 and N for VSMCs were 1.95 Gy,1.76 Gy,3.71 Gy and 2.47, respectively.The inhibitory effect of γ-ray irradiation on VSMCs proliferation was dose-dependent,being stronger along with increase of dose.VSMCs did not undergo apoptosis within 48 hours after γ-ray irradiation. Conclusion γ-ray irradiation could inhibit the proliferation of VSMCs,the main mechnism of which is the killing effect and inhibition of mitosis of VSMCs. |
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