| HE Pei,DING Zhenhua,ZHENG Li.Effects of combined radiation-burn injury on the serum level of IL-3 in mice[J].Chinese Journal of Radiological Medicine and Protection,2001,21(3):192-194 |
| Effects of combined radiation-burn injury on the serum level of IL-3 in mice |
| Received:August 04, 2000 |
| DOI： |
| KeyWords:Irradiation Burn Combined radiation-burn injury Interleukin-3 Mice Hematopoietic failure |
| FundProject:全军九五指令性课题资助项目(96L045) |
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| Abstract:: |
| Objective To explore the relationship between endogenous interleukin-3 and hematopoietic failure caused by combined radiation-burn injury. Methods Mice were randomized into normal (N),irradiation (R),burn (B) and combined irradiation-burn injury (C) groups.Serum level of IL-3 was detected by ELISA using the Endogenous Mouse IL-3 ELISA Kit at different times after injury. Results Compared with group N the serum level of IL-3 was significantly decreased in group R,especially on the 3rd and the 5th day (below the detecting sensitivity).It recovered slowly from the 15th day and did not elevate to the normal value on the 28th day.In group B.IL-3 was less than normal value on the 3rd and 5th day,then it increased rapidly and reached the peak (210% of that of the group N) on the 15th day.In group C,the serum level of IL-3 was higher than that of group R but lower than that of group B.The changes of the peripheral white blood cell in each group were parallel with the changes of IL-3.The serum of burnt mice collected from the 1st to 11 day showed inhibitory effect on CFU-F,stromal cell adhering rate of bone marrow stromal cells (BMSC),whereas serum harvested from the 13th to 15th days showed stimulation effect. Conclusion The serum of burnt mice collected at different times showing different actions on BMSC,and abnomal WBC caused by combined radiation-burn injury might be related with the changes in endogenous IL-3.The results of this experiment indicates:inhibition of endogenous IL-3 is one of the causes of hematopoietic failure induced by combined radiation-burn injury in mice. |
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