| SHI Qin,ZHANG Xueguang,XU Ying,et al.Effects of interleukin 6 on 60Co-γ-radiation induced apoptosis of multiple myeloma cell lines[J].Chinese Journal of Radiological Medicine and Protection,2001,21(3):188-191 |
| Effects of interleukin 6 on 60Co-γ-radiation induced apoptosis of multiple myeloma cell lines |
| Received:August 04, 2000 |
| DOI： |
| KeyWords:Interleuking 6 (IL6) Multiple myeloma cell lines γ-rays Apoptosis |
| FundProject:核工业预研基金资助项目(Y5573262) |
| Author Name | Affiliation | | SHI Qin | Institute of Biotechnology, Suzhou Medical College, Suzhou, 215007, China | | ZHANG Xueguang | Institute of Biotechnology, Suzhou Medical College, Suzhou, 215007, China | | XU Ying | Institute of Biotechnology, Suzhou Medical College, Suzhou, 215007, China | | 许志祥 | Institute of Biotechnology, Suzhou Medical College, Suzhou, 215007, China | | 强亦忠 | Institute of Biotechnology, Suzhou Medical College, Suzhou, 215007, China |
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| Abstract:: |
| Objective To investigate interleukin 6 involvement in the response of multiple myeloma cell lines XG-6 and XG-7 to 60 Coγ-radiation. Methods Apoptosis was confirmed by flow cytometry and quantitated by the assay of DNA fragmentation release.The expression of Bcl-xL and Bax in XG-6 and XG-7 cells after irradiation and cultured with or without interleukin 6 was monitored by RT-PCR. Results XG-6 and XG-7 cells in the presence or absence of interleukin 6 underwent apoptosis at 24 h after γ-irradiation,but in the absence of interleukin 6 they had higher rates of γ-radiation-induced apoptosis than in the presence of interleukin 6(P<0.05) and had lower levels of Bcl-xL and Bax expression. Conclusion Interleukin 6 affects the sensitivity of the cell lines to γ-radiation-induced apoptosis and can rescue cells from apoptosis by up-regulating the expression of Bcl-xL and changing the Bcl-xL/Bax ratio. |
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