| BIAN Huijie,CHEN Zhinan,LOU Chao.Cell cycle block and apoptosis in hepatocellular carcinoma cells induced by radionuclide 188Re[J].Chinese Journal of Radiological Medicine and Protection,2001,21(3):185-187 |
| Cell cycle block and apoptosis in hepatocellular carcinoma cells induced by radionuclide 188Re |
| Received:July 18, 2000 |
| DOI： |
| KeyWords:Cell cycle block Rhenium-188 Apoptosis Hepatocellular carcinoma cell β-ray |
| FundProject:国家自然科学基金资助项目(39700175) |
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| Abstract:: |
| Objective To study radionuclide 188 Re-induced cell cycle block and apoptosis in human hepatocellular carcinoma cells. Methods Cell morphology,agarose gel electrophoresis,and flow cytometry were used to examine 188 Re-induced cell cycle block and apotosis in human hepatocellular carcinoma cells. Results Dying cells induced by 188 Re displayed typical morphological and biochemical changes of apoptosis,Flow cytometry demonstrated the changeable response in various phases of the cell cycle.The apoptotic percentage depended on the radiation dose. Conclusion The effects of high and low dose of 188 Re-β-rays on the HCC cell cycle are different,for instance,low dose results in cell cycle block at G 0/G 1 phase,however high dose makes cells to escape G 0/G 1 arrest and increase their number at S and G 2/M phases. |
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