KONG Peiyan,LUO Chengji,GUO Chaohua.Preliminary study on expression of CD34 in murine bone marrow cells with radiation injury and its significance[J].Chinese Journal of Radiological Medicine and Protection,2001,21(2):117-120 |
Preliminary study on expression of CD34 in murine bone marrow cells with radiation injury and its significance |
Received:May 20, 2000 |
DOI: |
KeyWords:Irradiation Expression of CD34 antigen Bone marrow Mononuclear cells |
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Author Name | Affiliation | KONG Peiyan | Institute of Combined Injury, The Third Military Medilcal College, Chongqing 400038, China | LUO Chengji | Institute of Combined Injury, The Third Military Medilcal College, Chongqing 400038, China | GUO Chaohua | Institute of Combined Injury, The Third Military Medilcal College, Chongqing 400038, China | 周燕虹 | Institute of Combined Injury, The Third Military Medilcal College, Chongqing 400038, China | 邹仲敏 | Institute of Combined Injury, The Third Military Medilcal College, Chongqing 400038, China | 施炎 | Institute of Combined Injury, The Third Military Medilcal College, Chongqing 400038, China |
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Abstract:: |
Objective To analyses the expressional change of the CD34 in bone marrow hematopoietic cells postirradiation. Methods Female Kunming strain mice irradiated with 6 Gy-γrays without any treatment were used to analyse the CD34 expression in bone marrow mononuclear cells at 24 h,day 3,5,7,10,12 and 15 after irradiation.Collected bone marrow mononuclear cells were used to extract the total RNA.Using the methods of RT-PCR and dot blot hybridization with mouse CD34 specific primers and probe to measure the relative expression quantity. Results The CD34 expression was obviously increased as soon as 24 h post-irradiation.Afterwards,it maintained at a higher level than normal until day 15. Conclusion The results indicate that after 6 Gy irradiation,the earlier hematopoietic progenitors can increase the irproliferative ability and the quantity compensatively,and would have important significance in hematopoietic repair postirradiation. |
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