| WANG Yanjiang,SU Yongping,AI Guoping.Influence of total body irradiation on IL-12 expression in murine macrophages[J].Chinese Journal of Radiological Medicine and Protection,2001,21(2):96-99 |
| Influence of total body irradiation on IL-12 expression in murine macrophages |
| Received:August 20, 2000 |
| DOI： |
| KeyWords:Radiation Cell mediated immunity Macrophage IL-12 |
| FundProject:全军九五攻关课题基金资助项目(96L045) |
| Author Name | Affiliation | | WANG Yanjiang | Institute of Combined Injury, Third Military Medical University, Chongqing 400038, China | | SU Yongping | Institute of Combined Injury, Third Military Medical University, Chongqing 400038, China | | AI Guoping | Institute of Combined Injury, Third Military Medical University, Chongqing 400038, China | | 冉新泽 | Institute of Combined Injury, Third Military Medical University, Chongqing 400038, China | | 刘晓宏 | Institute of Combined Injury, Third Military Medical University, Chongqing 400038, China | | 袁良平 | Institute of Combined Injury, Third Military Medical University, Chongqing 400038, China | | 程天民 | Institute of Combined Injury, Third Military Medical University, Chongqing 400038, China |
|
| Hits: 2813 |
| Download times: 1867 |
| Abstract:: |
| Objective To investigate the influence of total body irradiation(TBI)on the cell count,morphology and IL-12 transcriptive level of peritoneal macrophages(pM¢s). Methods At different timepoints post TBI,pM¢s were purified,counted and observed under microscope,and the transcriptive level of IL-12 subunits was detected with RT PCR. Results After irradiation:①pM¢s decreased on days 3 and 7,then partially recoveved on days 15 and 30;②pM¢s looked like polymorphic and fibriform;③IL-12 p35 transcription was suppressed while that of p40 was elevated;④the higher the dose,the more serious the damage and the slower its recovery.The damage was predominant within 7 days,while the recovery become evident beginning from day 15 after radiation. Conclusion Reduction and transfiguration of pM¢s,suppression of IL-12 p35 transcription and elevation of IL-12 p40 transcription might be important contributors of immunity suppression.Only combination of p35 transcription with that of p40 should be used appropriately to evaluate the state of IL-12 expression after irradiation. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
