| LOU Tiezhu,GE Shili,XIANG Xiaoqiong,et al.Mutation of p53 gene in human bronchial epithelial cells BEP2D induced by α-particles[J].Chinese Journal of Radiological Medicine and Protection,2001,21(2):93-95 |
| Mutation of p53 gene in human bronchial epithelial cells BEP2D induced by α-particles |
| Received:July 11, 2000 |
| DOI： |
| KeyWords:α-particles BEP2D Cell transformation p53 PCRSSCP |
| FundProject:国家重点基础研究专项经费资助项目(G1998051200) |
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| Abstract:: |
| Objective To detect p53 gene mutation in BEP2D cells induced by α-particles.Methods Analysis of genomic DNA from the transformed cells using PCR amplification and single strand conformation polymorphism (SSCP).Results SSCP and restriction enzyme analysis demonstrated a point mutation at codon 249 of tumor suppressor gene p53 which has been thought to be the “hotspot” of mutation in transformation induced by α particles.Southern blot analysis showed a deletion in exon 5/6 of p53 gene,but there was no mutation in the exon 8/9 of p53 gene.Conclusion p53 gene is the “target” of DNA lesions induced by α particles.It plays an important role in the process of transformation of bronchial epithelial cells induced by α irradiation. |
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