| WANG Xiaoling,LI Jin,WANG Zhiquan.Fluorescence in situ hybridization detection of chromosome translocations induced by 60Coγ-rays in human lymphocytes dose-response curve and persistence[J].Chinese Journal of Radiological Medicine and Protection,2001,21(2):83-86 |
| Fluorescence in situ hybridization detection of chromosome translocations induced by 60Coγ-rays in human lymphocytes dose-response curve and persistence |
| Received:April 20, 2000 |
| DOI： |
| KeyWords:60Co γ-radiation Fluorescence in situ hybridization Translocation Doseresponse curve Persistence |
| FundProject:中日协作课题;院校科研基金资助项目(952060) |
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| Abstract:: |
| Objective To study doseresponse relationship for chromosome translocation and its persistence measured in human lymphocytes exposed to 60Co γ-rays.Methods The chromosome translocations in human peripheral lymphocytes were detected by fluorescence in situ hybridization using 4# and 7# combination of composite whole chromosomespecific DNA probes and Giemsa stain.Results The doseresponse relationship for chromosome translocation induced by 60Co γ-rays in vitro could be described by the function:Y=0.0030+0.0134D+0.0165D2.The frequencies of chromosome translocations induced by 0.5 and 2 Gy γirradiation did not diminish over time,so it exhibited excellent persistence.Conclusions The results indicate that retrospective dosereconstruction can be accomplished using chromosome translocation frequency. |
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