| XIANG Yingsong,YANG Rujun,TANG Gusheng.Characterization of death of human fetal bone marrow CD34+ cells after different dose of γ-irradiation[J].Chinese Journal of Radiological Medicine and Protection,2001,21(2):73-75 |
| Characterization of death of human fetal bone marrow CD34+ cells after different dose of γ-irradiation |
| Received:August 25, 2000 |
| DOI： |
| KeyWords:Hematopoietic stem cell apoptosis reproductive death niche |
| FundProject:国家自然科学基金资助项目(39770329,39600077) |
| Author Name | Affiliation | | XIANG Yingsong | Department of Radiation Medicine of Second Military Medical University, Shanghai 200433, China | | YANG Rujun | Department of Radiation Medicine of Second Military Medical University, Shanghai 200433, China | | TANG Gusheng | Department of Radiation Medicine of Second Military Medical University, Shanghai 200433, China | | 许熊飞 | Department of Radiation Medicine of Second Military Medical University, Shanghai 200433, China | | 张明辉 | 第二军医大学免疫室 | | 刘书逊 | 第二军医大学免疫室 | | 蔡建明 | Department of Radiation Medicine of Second Military Medical University, Shanghai 200433, China | | 杨平 | Department of Radiation Medicine of Second Military Medical University, Shanghai 200433, China | | 曹雪涛 | 第二军医大学免疫室 |
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| Abstract:: |
| Objective To investigate the characterization of death of the human hematopoietic stem cells after irradiation. Methods Human fetal bone marrow mononuclear cells were irradiated with different doses of 60Co γ-rays at different high dose rates.Apoptosis and necrosis of CD34+ cells were analyzed by flow cytometry,following threecolor labeling with PE-CD34/FITC-Annexin V/7AAD at different times after irradiation. Results The death of CD34+ cells after 5 Gy and 8 Gy irradiation showed a continuous process of reproductive death during the first week,and the main death type was apoptosis.A majority of CD34+ cells died of necrosis during the first day after 10 Gy and 12 Gy irradiation,and all of them died within a week. Conclusion Niches are continuously vacated every day within a week following irradiation and reproductive death of hematopoietic stem cells occurred. |
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