XUE lixiang,JIN Guanghui,FU Shibo,et al.Studies on apoptosis and necrosis of EL-4 cells after irradiation with different doses of X-rays[J].Chinese Journal of Radiological Medicine and Protection,2001,21(1):24-26
Studies on apoptosis and necrosis of EL-4 cells after irradiation with different doses of X-rays
Received:February 12, 2000  
DOI：
KeyWords:Irradiation  Apoptosis  Necrosis
FundProject:卫生部科学研究基金资助项目(981202)
Author NameAffiliation
XUE lixiang MH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun 130021, China 
JIN Guanghui MH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun 130021, China 
FU Shibo MH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun 130021, China 
苏旭 卫生部工业卫生实验所 
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Abstract::
      Objective To study the changes in apoptosis and necrosis of EL-4 cells following irradiation with different doses of X-rays. Methods The cells were stained by PI and Hoechst 33342 six hours after irradiation,and the changes in apoptosis and necrosis were analyzed by flow cytometry. Results It was found that apoptosis and necrosis increased significantly after high dose irradiation. Conclusion The results indicate that irradiation with certain doses can not only induce apoptosis but also cause necrosis at the same time.
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