| MA Zengchun,GAO Yue,LIU Yongxue.The changes of CD34+ cells in C57 mouse bone marrow after irradiation and their roles in dysfunction of hematopoiesis[J].Chinese Journal of Radiological Medicine and Protection,2001,21(1):12-15 |
| The changes of CD34+ cells in C57 mouse bone marrow after irradiation and their roles in dysfunction of hematopoiesis |
| Received:May 15, 2000 |
| DOI： |
| KeyWords:Irradiation Bone marrow Apoptosis CD34 Cell cyclX |
| FundProject:国家自然科学基金资助项目(39770887) |
| Author Name | Affiliation | | MA Zengchun | Department of Pharmacology and Toxicology, Institute of Radiation Medicine, Beijing 100850, China | | GAO Yue | Department of Pharmacology and Toxicology, Institute of Radiation Medicine, Beijing 100850, China | | LIU Yongxue | Department of Pharmacology and Toxicology, Institute of Radiation Medicine, Beijing 100850, China | | 谭洪玲 | Department of Pharmacology and Toxicology, Institute of Radiation Medicine, Beijing 100850, China | | 张立 | Department of Pharmacology and Toxicology, Institute of Radiation Medicine, Beijing 100850, China | | 陶来宝 | Department of Pharmacology and Toxicology, Institute of Radiation Medicine, Beijing 100850, China | | 陈鹏 | Department of Pharmacology and Toxicology, Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract:: |
| Objective To observe the changes of CD34 + cells in C57 mouse bone marrow after irradiation and investigate the role of apoptosis in radiation-induced dysfunction of hematopoiesis. Methods Flow cytometric enumeration of CD34 + hematopietic stem and progenitor cells by double fluorescent labeling apoptosis detection by Annexin V-FITC kit,and cell cycle detection by PI labeling were carried out. Results ①Compared with the normal group,the percentage of CD34 + cells in bone marrow nucleated cells decreased at least for 14 days after irradiation,and the changes were related with irradiation doses.②At 6 h after irradiation,the largest amount of apoptopic cells could be detected.③Bone marrow cell cycle was perturbed after 5.5 Gy irradiation. Conclusion The percentage of CD34+ hematopietic stem and progenitor cells in C57 mouse bone marrow decreased after irradiation,and apoptosis might be responsible for the changes of the bone marrow cells. |
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