| JIA Guanghong,MA Yexin,YU Shiying.Effect of γ-radiation on proliferation and apoptosis of smooth muscle cells[J].Chinese Journal of Radiological Medicine and Protection,2000,20(6):398-399 |
| Effect of γ-radiation on proliferation and apoptosis of smooth muscle cells |
| Received:September 09, 1999 |
| DOI： |
| KeyWords:Radiation Smooth muscle Apoptosis Proliferati |
| FundProject:湖北省科委九五攻关资助项目(962P101) |
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| Abstract:: |
| Objective To study the molecular mechanism of endovascular brachyterapy to reduce restenosis after coronary artery balloon angioplasty. Methods The cultured vascular smooth muscle cells (VSMC) were irradiated with 3.5, 7, 14, 28 Gy 60Co γ rays, respectively. The cell cycle and apoptosis of smooth muscle cells (SMC)were determined by flow cytometry at 12 h after brachytherapy. Results The percentages of G0-G1 phases were 58.13%, 83.6%, 88.79% and 89.15%, respectively in irradiation groups.Compared with the control group (56.29%), the proliferation of SMC was inhibited significantly by irradiation with 7, 14, 28 Gy 60Co γ rays (P<0.01) .The percentages of apoptosis were 0.85%, 1.04%, 10.38%, 16.00% in irradiation groups, respectively. Compared with the control group (0.84%), apoptosis was induced significantly by 14, 28 Gy γ irradiation (P<0.01) . Conclusion The irradiation dose of 3.5 Gy 60Co γ rays can neither inhibit proliferatrion nor induce apoptosis of SMC, 7 Gy can inhibit proliferation, but cannot induce apoptosis, whereas 14, 28 Gy can both inhibit proliferation and induce apoptosis of SMC. |
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