| YANG Zhanshan,ZHU Shoupeng,YANG Shuqin.Effects of in vivo exposure to low doses of different radiator nuclides on DNA repair capability[J].Chinese Journal of Radiological Medicine and Protection,2000,20(6):395-397 |
| Effects of in vivo exposure to low doses of different radiator nuclides on DNA repair capability |
| Received:April 17, 2000 |
| DOI： |
| KeyWords:235UO2F2 147Pm Lymphocytes Germ cells UDS |
| FundProject:国家核工业科学基金资助项目(71966201) |
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| Abstract:: |
| Objective To elucidate the effects and mechanism of in vivo exposure to low doses of different radiator nuclides on DNA repair capability. Methods The DNA excision repair capacity, as measured by UV-induced unscheduled DNA synthesis (UDS), of splenic lymphocytes and germ cells from inbred BALB/c male mice was observed after injection of different dose of 235UO2F2 or 147Pm(NO3)3 into tailvein. Results UV-induced UDS of the splenic lymphocytes was significantly increased (P<0.05 or 0.01) when the mice were exposed to 235UO2F2 at doses of 0.1-20 μg/kg body weight or 147Pm at doses of 37～185 Bq/g body weight, and UV-induced UDS of the germ cells was also increased at above doses of 147Pm (P<0.05 or 0.01).While the UV-induced UDS of these cells was decreased at doses of 3 700 kBq/g body weight of 147Pm (P<0.05 or 0.01).Non-UV-induced UDS showed a significant increase on the 12th day after 235UO2F2 injection(P<0.05 or 0.01).The values of UV-induced UDS in PHA activated but non-proliferating (hydroxyurea treated) lymphocytes were lower than those of lymphocytes unexposed to PHA at the same doses of 235UO2F2(P<0.05 or 0.01). Conclusion Low doses of internally deposited 235UO2F2 and 147Pm have a continuous damage effect on DNA of the murine somatic and germ cells so that a significant stimulative effect on DNA excision repair of the cells is induced.But higher doses of 147Pm irradiation have a significant inhibitory effect on DNA repair of those cells.The stimulative effect occurs only in a limited range of dose.In PHA stimulated but non-proliferating lymphocytes, the DNA repair synthesis is significantly inhibited after UV-irradiation. |
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