| CHEN Xiaohong,CHENG Tianmin,RAN Xinze.Effects of irradiation on actin in wound neutrophils of rats[J].Chinese Journal of Radiological Medicine and Protection,2000,20(6):388-390 |
| Effects of irradiation on actin in wound neutrophils of rats |
| Received:September 08, 1999 |
| DOI： |
| KeyWords:Wound combined with radiation injury Neutrophils Actin |
| FundProject:重庆市科委院士基金资助项目(985081);国家重点基础研究973专项经费资助项目(G1999054205) |
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| Abstract:: |
| Objective To explore the mechanism of decreased phagocyotic and chemotactic function of neutrophils (Neu) and the mechanism of W11-a12' s pharmacological action by studying the changes of actin of Neu in wound after total body irradiation of rats. Methods Cells were collected from incision wounds on back of rats after 6 Gy whole body-irradiation.F-actin in Neu was stained with Fluorescein-phalloidin. Results There were increased (3～12 h) and decreased (24 h, 48 h) basal levels of F-actin, a lower responsiveness to stimulation and a reduced rate of actin depolymerization after combined radiation injury compared with simple wound group, which were important causes of Neu dysfunction. Conclusion W11-a12 could improve the functional state of Neu actin, which may contribute to the improved chemotactic and phagocytotic function of Neu after administration of this drug. |
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