| FU Haiqing,JU Guizhi,LUO Can.The role of p21 in G1 arrest of EL-4 cells induced by ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2000,20(6):385-387 |
| The role of p21 in G1 arrest of EL-4 cells induced by ionizing radiation |
| Received:November 12, 1999 |
| DOI： |
| KeyWords:Ionizing radiation G1 arrest p21 |
| FundProject:国家自然科学基金资助项目(39770193) |
| Author Name | Affiliation | | FU Haiqing | MH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun 130021, China | | JU Guizhi | MH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun 130021, China | | LUO Can | MH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun 130021, China | | 傅士波 | MH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun 130021, China |
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| Abstract:: |
| Objective To elucidate the role of p21 in G1 arrest of EL-4 cells induced by ionizing radiation. Methods Northern blot was used to measure p21 WAF1 mRNA level.Flow cytometry was employed to analyze p21 protein expression and cell cycle of EL-4 cells. Results Following 4.0 Gy X-irradiation, EL-4 cells in G1 phase began to significantly increase at 12 h, reached the peak at 24 h, and kept at high level till 48 h;the p21 WAF1 mRNA level began to increase at 1 h, reached the peak level at 4 h, kept the high levels till 12 h, then recovered to normal at 24 h;and the level of p21 protein expression in EI-4 cells increased significantly at 2 h to 48 h. Conclusion The results suggest that p21 might play an important role in G1 arrest of EL-4 cells induced by ionizing radiation. |
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