| ZHU Bo,LUO Chengji,GUO Chaohua.Changes of nuclear factor-κB (NF-κB) in cultured bone marrow stromal cells after 60Coγ-irradiation[J].Chinese Journal of Radiological Medicine and Protection,2000,20(5):317-320 |
| Changes of nuclear factor-κB (NF-κB) in cultured bone marrow stromal cells after 60Coγ-irradiation |
| Received:December 24, 1999 |
| DOI： |
| KeyWords:Bone marrow stromal cells Radiation Bone marrow hematopoietic microenvironment NF-κB |
| FundProject:全军“九五”指令性公关资助项目(P6L045) |
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| Abstract:: |
| Objective To explore the changes in bone marrow hematopoietic microenvironment after exposure to γ-radiation. Methods Immunohistochemistry,Western blot and electrophoretic mobility shift assay (EMSA)was used. Results The nuclear translocation was found in cultured bone marrow stromal cells after 60 Coγ-irradiation with immunohistochemistry.The expression of NF- κB in the bone marrow stromal cells (BMSCs) on the level of protein was elevated after exposure to 60 Coγ-rays at the dose of 8. 0Gy with the uses of immunohistochemistry and Western blot.The activities of NF-κB in the BMSCs increased significantly after exposure to γ-radiation by using EMSA.The activity peak was at 4 hours after irradiation. Conclusion Our results suggest that the activation of NF-κB in the BMSCs by irradiation might be involved in the protection of BMSCs and in the recovery of hematopoiesis after γ-irradiation. |
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