| XU Yongzhong,ZHENG Siying,ZHAO Jingyong,et al.Dose response curves for chromosome aberrations induced by 60Coγ-rays and analysed by dual colour fluorescence in situ hybridization[J].Chinese Journal of Radiological Medicine and Protection,2000,20(4):254-256 |
| Dose response curves for chromosome aberrations induced by 60Coγ-rays and analysed by dual colour fluorescence in situ hybridization |
| Received:October 30, 1999 |
| DOI： |
| KeyWords:Fluorescence in situ hybridization Translocation Dicentric Dose response relationship |
| FundProject:国防科工委基金资助项目(Y5573262) |
| Author Name | Affiliation | | XU Yongzhong | Department of Radiation Medicine, Soochow University, Suzhou 215007, China | | ZHENG Siying | Department of Radiation Medicine, Soochow University, Suzhou 215007, China | | ZHAO Jingyong | Department of Radiation Medicine, Soochow University, Suzhou 215007, China | | 曹建平 | Department of Radiation Medicine, Soochow University, Suzhou 215007, China | | 朱巍 | Department of Radiation Medicine, Soochow University, Suzhou 215007, China |
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| Abstract:: |
| Objective To explore the feasibility of using fluorescence in situ hybridization (FISH) to detect stable chromosome translocation as a biological dosimeter.Methods Translocation and dicentric frequencies in peripheral blood lymphocytes induced by 60Co γ-rays at different doses were analysed by conventional staining and dual colour FISHwith composite whole chromosome specific probes for human chromosomes 1 and 4, and their dose response curves were fitted. Results All dose response curves for translocation and dicentrics from FISHanalysis, as well as dicentrics from conventional staining could be described by linear quadratic equations Y=c+αD+βD2. The difference between dicentric frequencies analysed by FISHand conventional staining were not statistically significant ( P >0.05). For radiation induced translocation and dicentrics in chromosomes 1 and 4, no significant differences were found between the observed frequencies and expected frequencies based on DNAcontent.Conclusion Chromosome translocation can be quickly and accurately analysed by FISHand has a good dose response relationship; thus, it is hopeful to use translocation frequencies measured by FISHas a long term or accumulative biological dosimeter. |
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