| GAO Linlu,CUI Yufang,YANG Hong,et al.Expression of Fas and Bcl-2 and their relationship to apoptosis in spleen lymphocytes of mice irradiated with large dose 60Coγ-rays[J].Chinese Journal of Radiological Medicine and Protection,2000,20(4):252-253 |
| Expression of Fas and Bcl-2 and their relationship to apoptosis in spleen lymphocytes of mice irradiated with large dose 60Coγ-rays |
| Received:September 18, 1999 |
| DOI： |
| KeyWords:Irradiation Spleen lymphocytes Apoptosis Fas Bcl-2 |
| FundProject:解放军总后勤部留学回国人员启动基金资助项目(9405021);军事医学科学院第二届创新基金资助项目(9805106) |
| Author Name | Affiliation | | GAO Linlu | Institute of Radiation Medicine, Beijing 100850, China | | CUI Yufang | Institute of Radiation Medicine, Beijing 100850, China | | YANG Hong | Institute of Radiation Medicine, Beijing 100850, China | | 夏国伟 | Institute of Radiation Medicine, Beijing 100850, China | | 彭瑞云 | Institute of Radiation Medicine, Beijing 100850, China | | 高亚兵 | Institute of Radiation Medicine, Beijing 100850, China | | 王德文 | Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract:: |
| Objective To investigate the expressions of Fas and Bcl-2 and their significance in apoptosis of spleen lymphocyte of mice after large dose γ-ray irradiation.Methods At 3, 6, 12, 24 h, 3, 7, 14 and 28 d after 6-20 Gy γ-ray irradiation mice were sacrificed and their spleens were removed.The expressions of Fas and Bcl-2 oncoprotein were analysed by LSABimmunohistochemical method. Results The expression of Fas was strongly positive at 6 h after irradiation, especially in 6-12 Gy groups. It became less obvious along with prolongation of time after irradiation and almost disappeared on d 7 after irradiation. The expression of Bcl-2 was nearly negative at 6 h after irradiation, especially in 12-20 Gy groups, and did not recover on d 28 after irradiation.Conclusion After large dose γ-ray irradiation the expression of Fas in mouse spleen lymphocytes shows a better relationship to lymphocyte apoptosis; in other words, Fas can prompt apoptosis. On the other hand, the action of Bcl-2 is reduced or even disappeared. Both of them play an important role in spleen lymphocyte apoptosis after large dose of γ-irradiation. |
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