| ZHU Maoxiang,YANG Zhihua,GONG Yifen,et al.Oxidative damage of BEP2D cells irradiated 60Coγ-rays[J].Chinese Journal of Radiological Medicine and Protection,2000,20(4):248-251 |
| Oxidative damage of BEP2D cells irradiated 60Coγ-rays |
| Received:September 26, 1999 |
| DOI： |
| KeyWords:60Co γ-ray irradiation HPV-16 immortalized human bronchial epithelial cell Reactive oxygen species 8-Hydroxydeoxygunosine Selenium |
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| Abstract:: |
| Objective To study reactive oxygen species (ROS) productions and oxidative DNAdamage in HPV-16 immortalized human bronchial epithelial cells(BEP2D) irradiated with 60Co γ-rays. Methods The measurement of extracellular superoxide anions (O 2) was based on the reduction of ferricytochrome Cas assayed by the increase in its absorbance at 550 nm.Quantitation of extracellular hydrogen peroxides (H2O2) was based on the horseradish peroxidase-dependent oxidation of phenol red which is assayed by its increased absorbance at 620 nm.The determination of extracellular hydroxyl radicals (·OH) was based on decoloration of safranine Tas assayed by the decrease in its absorbance at 520 nm.Ethidium bromide and 2’, 7’-dichlorofluorescein, fluorescent products of the membrane-permeable dyes-hydroethine and 2’, 7’-dichloroflurescin diacetate, were used to monitor the intracellular production of H2O2 and O 2 respectively by flow cytometry.8-hydroxydeoxygunosine (OH8dG) was examined with HPLC-ECDfrom extracted DNA. Results The ROSproduction including H2O2, O2 and ·OH, and DNAadduct OH8dGin60 Co γ-rays-irradiated BEP2D cells increased remarkably, and these increments could be inhibited effectively by 1 μmol/Lof selenium. Conclusion 60Co γ-irradiation causes oxidative damage to BEP2D cells, which can be protected by selenium. |
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