| ZHANG Zhanying,ZHU Xuezhen,ZHANG Lansheng,et al.Mechanism of low GVHD incidence in umbilical cord blood transplantation[J].Chinese Journal of Radiological Medicine and Protection,2000,20(4):245-247 |
| Mechanism of low GVHD incidence in umbilical cord blood transplantation |
| Received:October 19, 1999 |
| DOI： |
| KeyWords:Umbilical cord blood T cells Soluble factors Graft-versus-host disease |
| FundProject::国家自然科学基金资助项目(39970244);国家核工业基金资助项目(Y7197Q6206) |
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| Abstract:: |
| Objective To compare the immunological function between umbilical cord blood (UCB) and peripheral blood, and to explore the mechanism of low incidence of graft-versus host disease (GVHD) in UCBtransplantation. Methods The proliferation assay, NKcell killing activity assay and immunologic fluorescence double label technique were used to study four sear effect on Tcell immunologic function. Results UCBserum could reduce K562 cell activity, compared to that of adult serum.Furthermore, adult serum will enhanced the percentage of HLA DRexpression of Tcells on PHA-stimulated PBMC, whereas fetal serum has no such effect.Both adult and fetal serum enhanced mitogen-specific Tcell proliferation, the peak proliferative response could be reached with adult serum but not with fetal serum. Conclusion There must be some soluble factors secreted into serum, which play a role in Tcell activation and associate with the incidence of GVHD. |
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