| JIANG Guochun,YUAN Lizhen,WEI Kang,et al.ku gene mutation of radiosensitive mouse mammary carcinoma derived cell line SX-9[J].Chinese Journal of Radiological Medicine and Protection,2000,20(4):242-244 |
| ku gene mutation of radiosensitive mouse mammary carcinoma derived cell line SX-9 |
| Received:September 28, 1999 |
| DOI： |
| KeyWords:Radiosensitivity ku gene Gene mutation |
| FundProject:国家自然科学基金资助项目(39900031) |
| Author Name | Affiliation | | JIANG Guochun | Institute of Radiation Medicine Beijing, 100850, China | | YUAN Lizhen | Institute of Radiation Medicine Beijing, 100850, China | | WEI Kang | Institute of Radiation Medicine Beijing, 100850, China | | 郭学敏 | Institute of Radiation Medicine Beijing, 100850, China | | 张雪峰 | Institute of Radiation Medicine Beijing, 100850, China | | 贾向旭 | Institute of Radiation Medicine Beijing, 100850, China |
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| Abstract:: |
| Objective To investigate the ku gene status of radiosensitive cell line SX-9 and the effects of ku80 gene mutation on radiosensitivity of SX-9. Methods After comparing the ku70/80 gene status of SX-9 to SR-1 with molecular cloning, the survival fraction of SX-9 transfected with wild-type human ku80, SX-9 and SR-1 was investigated by clonogenic assay. Results Radiosensitive mouse mammary carcinoma-derived cell line SX-9 bore mutation in ku80 gene, and its survival fraction after γ-ray irradiation could be restored by transfection of wild-type human ku80 gene. Conclusions There are two mutation sites in ku80 gene in radiosensitive mouse mammary carcinoma-derived cell line SX-9, which could be the cause of its deficiency in repair of DNAdouble strand breaks. |
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